Freeze-fracture and Freeze-etching Study of Encystment of Phytophthora palmivora Zoospores
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چکیده
منابع مشابه
Negative Chemotaxis of Zoospores of the Fungus Phytophthora palmivora
347 Phytophthora palmivora zoospores are repelled by many low molecular weight cations. This negative chemotactic response occurs at a different threshold concentration for each cation, that for the most effective ion, H+, being 150 ,!AM. The effectiveness of different cations parallels their ionic conductivities, the most active cations having the highest conductivities. It is suggested that t...
متن کاملAdhesion of Phytophthora palmivora zoospores: detection and ultrastructural visualization of concanavalin A-receptor sites appearing during encystment.
The binding of concanavalin A (Con A) to the cell surface of zoospores and cysts of Phytophthora palmivora was studied by radiometry (125I-Con A), ultraviolet microscopy (fluorescein-Con A) and electron microscopy peroxidase-diaminobenzidine technique). Zoospores were found to secrete during the early stages of encystment a Con A-binding material susceptible to trypsin digestion. This glycoprot...
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As part of a study of the cell surface changes associated with the production of murine mammary tumor virus, the structure of the envelope of this virus has been examined by using freeze-fracture techniques. Both fracture and deep-etch surfaces were examined. The fracture faces contain 10-nm spheres comparable to those observed on fractured plasma membranes, although fewer in number. Surfaces e...
متن کاملMembrane fusion during secretion. A hypothesis based on electron microscope observation of Phytophthora Palmivora zoospores during encystment
Interpretation of freeze-fracture and thin-section results shows that fusion of the peripheral vesicle with the plasmalemma of a Phytophthora palmivora zoospore occurs at several discrete sites and results in the formation and expansion of a particle-free bilayer membrane diaphragm and in the appearance of a polymorphic network of membrane-bounded tunnels, the lumina of which are continuous wit...
متن کاملViewed by Freeze-Etching'
The indigenous microflora of soil were released from the soil materials and concentrated without the occurrence of growth by use of a blending-simple centrifugation procedure. The cell concentrate was then frozen-etched and viewed by transmission electron microscopy. Criteria were established for detecting microbial cells among the residual soil debris. The freeze-etching of the soil cell conce...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-102-1-149